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IN VIVO 31P NMR STUDIES ON CEREBRAL INFARCTION USING TOPICAL MAGNETIC RESONANCE (TMR):TIME COURSE OF HIGH ENERGY PHOSPHORUS COMPOUNDS CONTENT IN ISCHEMIC AND RECIRCULATED BRAIN Shoji Naruse 1 , Yoshiharu Horikawa 1 , Chuzo Tanaka 1 , Kimiyoshi Hirakawa 1 , Hiroyasu Nishikawa 2 , Izumi Koizuka 3 , Soshun Takada 3 , Hiroshi Watari 3 1Department of Neurosurgery, Kyoto Prefectural University of Medicine 2Department of Physiology, Kyoto Prefectural University of Medicine 3Department of Molecular Physiology, National Institute for Physiological Sciences pp.603-609
Published Date 1983/6/1
DOI https://doi.org/10.11477/mf.1406205140
  • Abstract
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Sequential metabolic changes in rat brain were monitored by in vivo measurement of 31P NMR spectra using a topical magnetic resonance (TMR) spectrometer, during the course of experimentally induced cerebral infarction and during its recovery by restoration of circulation.

There were several peaks in the spectrum of the normal rat brain ; the β-ATP, α-ATP and α-ADP with contribution from NAD+/NADH, γ-ATP and ,β-ADP, phosphocreatine, phosphodie-sters or 2, 3 DPG, inorganic phosphate and sugar phosphate with contribution from AMP.

The experimental cerebral ischemia was render-ed to 16 Wistar rats by the method of Pulsinelli and Brierley. The coagulation of the bilateralvertebral arteries did not show any changes in NMR spectra, but the subsequent ligation of bilateral common carotid arteries produced a decrease in the peak of ATP and phosphocreatine and a concomitant increase in the peak of inorganic phosphate. The tissue pH, which was calculated from the chemical shift of inorganic phosphate, declined. These changes occurred rapidly within a few minutes, being followed by a gradual increase in their degree after the ischemia.

Reinstatement of blood circulation to the cereb-rum were made by untying the ligature of common carotid arteries in 6 rats at 30 minutes after, in 6 rats at 60 minutes after and in 4 rats at 120 minutes after the ischemia. The reinstate-ment of the circulation resulted in a immediaterestoration of the peaks of ATP and phosphocre-atine and in a reduction of the peak of inorganic phosphate within a few minutes in all rats of 30 minutes ischemia and a half of rats of 60 minutes ischemia. Their spectra recovered to preinfarction patterns by 30 to 60 minutes after the recircula-tion. However, no recovery of spectra were shown in a remained half of rats of 60 minutes ischemia and in all of 120 minutes ischemia.

These experiments demonstrated that high energy phosphorus compounds decrease very rapidly after the ischemia, and that these changes were reversible if the restoration of the circula-tion was made at least 30 to 60 minutes periods of ischemia.


Copyright © 1983, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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