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CONCENTRATIONS OF MONOAMINES AND MONOAMINE METABOLITES IN CEREBROSPINAL FLUID DETERMINED BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION Kimihiro Yoshino 1 1Department of Clinical Neurochemistry, Institute for Neurobiology, Okayama University Medical School pp.1099-1106
Published Date 1982/11/1
DOI https://doi.org/10.11477/mf.1406205030
  • Abstract
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Using reversed-phase high-performance liquid chromatography with an electrochemical detection, I have developed a sensitive technique to measure monoamines and their metabolites in cerebrospinal fluid (CSF). The present method has been shown to offer simplicity and high sensitivity for the determination of dopamine (DA) and norepineph-rine (NE), as well as monoamine metabolites, in small amounts of human CSF.

The first 2 ml of CSF was obtained from 61 pa-tients (27 males and 34 females), aged from 15 to 88 years, with a variety of non-neurological dise-ases by lumbar puncture performed between 8: 45 a. m. and 4:20 p. m. CSF was collected in the lateral decubitus position before lumbar anesthe-sia for surgical treatment. Samples were immedia-tely frozen at-80℃ until assayed. None had any history of neurological or psychiatric illness.

Concentrations in lumbar CSF were 10.9±6.0 pg/m/(mean±LSD, n=22) for DA, 105.8±63.6pg/ ml (n=60) for NE, 30.5±1.6ng/ml (n=61) for homovanillic acid (HVA), 1. 8±1.2ng/ml (n=46) for 3,4-dihydroxyphenylacetic acid (DOPAC), 7.7±2.1 ng/m/(n=46) for 3-methoxy-4-hydroxyphe-nylglycol (MHPG) and 18. 8±10.9 ng/m/ (n=61) for 5-hydroxyindoleacetic acid ( 5 HIAA), respectively. While 5 HIAA concentrations in lumbar CSF ta-ken in the afternoon tended to be lower than those in the morning, MHPG in the afternoon was significantly higher than that in the morning. There were no sex differences in the concentrations of monoamines and their metabolites examined. There was a tendency for the concentrations of HVA and DOPAC to be lower in older subjects. A significant correlation was found among HVA, 5 HIAA and MHPG concentrations in lumbar CSF.

The present study suggests that a standardized condition for collecting CSF should be employed to compare the concentrations of monoamines and their metabolites across central nervous system disorders. Furthermore, in addition to the mea-surement of individual monoamine or monoamine metabolite level in CSF, future studies should be extended to include comparisons of a mutual re-letionshin among several monoamine metabolites.


Copyright © 1982, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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