パール氏髓鞘染色法と併用し得る神經細胞の新しい染色法について

A New Method for Staining Nissl Bodies and other Structures of the Nervous Tissue on Materials fixed in the Müller's Solution.

緒論

　余は數年來東大解剖學教室及び腦研究室において多數のパール氏法による髓鞘染色標本を觀察してきたが，その際正常及び病的の人腦や實驗した動物腦を觀察するに神經纖維の他に同時に神經細胞，神經膠，血管等の變化をもよく見得る方法がないものかと常に考えていた。

　パール氏法は髓鞘を染める方法としてはまことに優秀であり，細い神經纖維の變化までかなりよく之によつて示される。然しNisslが"Faseranatomie keine Hi-stopathologie"と喝破した如く神經纖維の變化のみでは病理組織學の研究にならない。そのため病理組織學では神經細胞の變化を見るためニッスル法が主として用いられてきた。ところがこの方法では神經細胞神經膠の核はよく見えるが神經纖維や神經膠纖維の樣子が分らない。

The present author has been looking for a staining technic that would allow one to stain in serial sections of the brain the myelin shea-ths and the cells alternately, for the materi-als at first fixed in formalin and Müller's solution do not stain well by the Nissl's me-thod. Recently a new method was found out which seems to be very close to the ideal for this purpose.

Method.

1.Celloidin sections fixed in formalin and Müller's solution are washed in two chan- ges of distilled water.

2.Placed in Oxyful solution (3% of H₂O₂) from 10 to 60 minutes.

3.Washed in distilled water.

4.Placed then in following solution from 5 to 60 minutes.

a) Picric acid, saturated aqueous solution ………………………………… 20c.c.

b) Ice acetic acid………………………………80c.c.

5.Washed in distilled water, then in tap water.

6.Stained from 1 to 5 hours in Mayer's he- matoxylin at 37℃.

7.Washed in distilled water, then in tap water.

8.Transferred to 70 per cent, 90 per cent and absolute alcohol.

9.Cleared in carbol-xylol and transferred to xylol.

10.Mounted in balsam.

Results. This method gives better results than Nissl's in the following points.

1.The stained preparations are not so sensi- tive to sunlight and heat, do not fade out as quick as those stained after Nissl. It requires no greater care in handling and with a little practice yields very gratifying results.

2.Nissl bodies and nuclei.of the cells are as clear as those stained with Nissl's method.

3.It is more advantageous for the study of pigment granules.

4.It will find application in studies of non- myelinated nerve fibers.

5.It will allow one to stain fatty substances.