Carbohydrate Expression of Cancer Cells in Progression of Gastric Cancer from Early to Advanced Stage Takanori Kawaguchi 1 , Yoshinao Takano 2 , Takeo Ohori 3 , Fujio Ito 3 , Shin Koyama 3 1The Second Department of Pathology, Fukushima Medical College 2Department of Surgery, Southern Tohoku Research Institute of Neuroscience 3The First Department of Surgery, Fukushima Medical College Keyword: 胃癌 , 糖鎖抗原 , 浸潤・転移 , 予後 , p53蛋白 pp.797-808
Published Date 1997/5/25
DOI https://doi.org/10.11477/mf.1403105141
  • Abstract
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 We investigated the relationship between the progression of gastric cancer from early to advanced stage and the expression of carbohydrate antigens of cancer cells. Formalin-fixed and paraffin embedded sections from approximately 400 cases of primary gastric cancer were used. The detection of carbohydrate antigens was performed by avidin-biotin-peroxidase complex (ABC) methods using 17 kinds of biotin-labeled lectins (GS-I-B4, Jacalin, MPA, HPA, VVA, SBA, DBA, RCA-1, RCA-2, ECA, Lotus, AAA, UEA-1, PHA-L, WGA, ConA), anti-T (Thomsen-Friedenreich antigen), Tn, sialyl Tn antibodies, anti-blood group type A, B, and H (2) antibodies, and anti-Lewisx and anti-sialyl Lewisx antibodies. The expression of p53 protein was also investigated by immunohistochemistry using BP53-12 and PAb240 monoclonal antibodies. The results indicated that the carbohydrate antigen expression is profoundly associated with the progression of gastric cancer, based on either immature type of carbohydrates such as T and Tn antigens or mature type of carbohydrates such as ABH blood group antigens and Lewisx antigens. The former was related to the depth of invasion and lymph node metastasis, whereas the latter was related to hepatic and peritoneal metastases. The p53 protein expression was correlated positively with Tn antigen expression, especially in poorly differentiated type of gastric cancer. These results suggest that the carbohydrate deviations of cancer cells are one of the major causes of the progression of gastric cancer from early to advanced stage. The carbohydrate deviations may be due to p53 mutation in gastric cancer cells in part.

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