HPV-DNA tests available in Japan Toshiyuki Sasagawa 1 1Department of Obstetrics and Gynecology, Kanazawa Medical University Keyword: ヒトパピローマウイルス , 子宮頸癌 , HPV-DNA検査 , Hybrid Capture® , PCR pp.1433-1443
Published Date 2011/11/15
DOI https://doi.org/10.11477/mf.1542102839
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Human papillomavirus (HPV) types were classified with genome sequence homology, and more than 189 HPV types were identified. Thus, the presence of HPV infection is able to be detected by DNA testing. Two HPV-DNA tests called Hybrid capture, generation 2 (HC-2) and Amplicore HPV are commonly used for cervical cancer screening. The former test is based on the hybridization method, and the latter is based on the polymerase-chain-reaction (PCR) method. Both tests show positive if either of 13 high-risk HPV types including HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68 are detected. On the other hand, HPV genotyping tests available in Japan are the PCR-based, and the loop mediated isothermal amplification (LAMP)-based methods. It is important to know the merits and demerits of each HPV test in clinical settings, since inappropriate use of the HPV-test may lead to miss-diagnosis and over-treatment.

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