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Inhibitory effect of okadaic acid on protein phosphatases; characteristics and applications. Akira TAKAI 1 1Department of Physiology, School of Medicine, Nagoya University pp.575-583
Published Date 1991/8/10
DOI https://doi.org/10.11477/mf.1431900158
  • Abstract
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Okadaic acid is the first exogenous substance that has been described to have a potent and specific inhibitory effect on protein phosphataes. The dissociation constants for the interaction of okadaic acid wih type 2A-, type 1- and type 2B-protein phosphatases are 30pM, 300nM and 5μM respectively. Enzyme-kinetic studies have shown that okadaic acid acts as a non-competitive or mixed inhibitor on these okadaic acid-sensitive phosphatases, which are structurally related enzymes, having 50% amino acid sequence homology in the catalytic domain. The following enzymes are not affected by up to 10 μM okadaic acid: type 2C-protein phosphatase, phosphotyrosyl phosphatase, inositol-1, 4, 5-trisphosphate phosphatase, acid phosphatases, alkaline phosphatases, Ca2+/calmodulin-dependent phosphodiesterase, myosin light-chain phosphatase, cAMP-dependent protein kinase and protein kinase C. Because of the especially high affinity for type 2A-phosphatase, combined use of okadaic acid with inhibitor 2, an intrinsic inhibitory factor of type 1-phosphatase, provide a new method for analysing the protein phosphatase composition in a relatively small amount of tissue extract. Okadaic acid, being membranepermeable, can effectively be used for investigating possible roles of protein phosphorylation in regulation of ionic channels.


Copyright © 1991, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1243 印刷版ISSN 0001-8724 医学書院

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