冷凍保存角膜による角膜移植について

Experimental Studies of Keratoplasty with Coneas and Eyes Preserved by Deep Freezing

緒言

　1958年本邦に於ても角膜移植法が成立し，今後は移植角膜採取に法律的な見解が明白になつた。

　死体眼球を用いる同種角膜移植はFilatov^1）（1929〜1932）が始めて成功を収め，本邦に於ては中村^2）−7）（1940）による研究に於て，0°〜6℃で血清，リンゲル液，生食水に保存して，特に血清内貯蔵が勝れて居り，又湿潤した空気中に貯えて5日間透明を保つた。同様の方法で北川^8）は24時間以内のものが成績が良く，Patton^9）は72時間以内は使用出来ると云つており，又Holtt^10）は48時間より古いものは用いるべきでないと主張し，Stocker^11）も24時間以内に用いねばならぬと述べて居る。

The viability of the cornea preserved under extremely low temperature, following the frozen preservation method of bovine sperms introduced by Polge et al., was investigated. The eye-ball enucleated from the dead cat was placed in the moist air of 4℃ for 24 hours. Then the eye-ball or its cornea was immersed in glycerine for 2 hours and was kept in a glass test tube (shown in the picture) in the deep freezer at -80℃ for one week to 3 months. After the preservation, the eye-ball or cornea was rapidly thawed and used for experimental keratoplasty.