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A CELL KINETIC STUDY OF HUMAN CENTRAL NERVOUS SYSTEM TUMORS WlTH 5-BROMODEOXYURIDINE (BrdU) Tadashi Nagashima 1 , Takao Hoshino 1 1Department of Neurosurgery, University of California pp.1195-1201
Published Date 1985/12/1
DOI https://doi.org/10.11477/mf.1406205629
  • Abstract
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Sixty-six patients with various types of central nervous system (CNS) tumors were given 150-200 mg/m2 of 5-bromodeoxyuridine (BrdU) intrave-nously at the start of surgery, in order to label DNA synthesizing cells in the tumor with BrdU. The excised tumor specimens were fixed in chilled 70% ethanol for at least 12 hours, embedded in paraffin, and cut into 6-8 it thick sections. The tissue sections were deparaffinized, denatured with HCI, and stained by indirect peroxidase method using anti-BrdU monoclonal antibody as the first antibody. The percentage of labeled cells among total cells (LI: labeling index) was calculated in each tumor specimen.

The average of LIs was over 10% in glioblas-toma multiforme, medulloblastoma, and metastatictumor. On the other hand, the LIs were very low, i.e.less than 1% in most cases of ependymomas, mixed gliomas, and moderately anaplastic astrocy-tomas. In highly anaplastic astrocytomas, the average of LIs resulted in 7.4%. The LIs of slow-growing tumors, such as pituitary adenomas, meningiomas, acoustic neurinomas, were less than 1%, except in Nelson's syndrome and a few cases of meningiomas. The LIs obtained thus seem to correlate with the clinical behavior of each type of tumor. However, there was still a magnitude of differences in LIs between the tumors which were histopathologically alike.

These results indicate that the LI obtained by the immunohistochemical method using anti-BrdU monoclonal antibody may supplement the current histopathological diagnosis in characterizing as well as estimating the biological malignancy of individual CNS tumors.

This work was supported in part by grants PDT-159 from ACS and CA 13525 from NCI.


Copyright © 1985, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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