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Fluid shear stress effects on intracellular calcium concentrations in cultured vascular endothelial cells Joji Ando 1 , Akira Ohtsuka 1 , Youichi Katayama 1 , Shigenobu Araya 1 , Akira Kamiya 2 1Research Institute of Applied Electricity, Hokkaido University 2Institute for Medical Electronics, Faculty of Medicine, The University of Tokyo Keyword: 血行力学的応力(hemodynamic force) , 流れずり応力(Huid shear stress) , 血管内皮細胞(vascular endothelial cell) pp.1107-1113
Published Date 1990/11/15
DOI https://doi.org/10.11477/mf.1404910044
  • Abstract
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Vascular endothelial cells are known to modulate their functions in response not only to humoral stimuli but also to such physical stimuli as fluid shear stress generated by blood flow. However, the mechanisms by which the hemodynamic force acts on endothelial cells are not yet well understood. We have studied how endothelial cells recognizethe shear stress and mediate it to intracellular or-ganelles. Cultured monolayers of bovine aortic en-dothelial cells loaded with the highly fluorescent Ca++-sensitive dye Fura 2 were exposed to different levels of fluid shear stress in a specially designed flow chamber and simultaneous changes in intracel-lular-free Ca++ concentration were measured using photometric fluorescence microscopy.

Application of shear stress to cells by fluid perfu-sion led to an immediate several-fold increase in Ca++ concentration within 1 min, followed by a rapid decline, and finally a plateau somewhat higher than control levels during the entire period of the stress application. The early part of the response, but no plateau, was observed even in Ca++-free me-dium added with 2mM EDTA, and in the presence of calcium antagonists (e. g. 2x10-5M nicardipine), Thus, endothelial cells may have a flow-sensing property which recognize the shear stress on the membrane as a stimulus and mediates the signal to increase intracellular free Ca++ which is a major component of the internal signalling system of the cell.


Copyright © 1990, Igaku-Shoin Ltd. All rights reserved.

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