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要旨
西別府病院における2000年から2002年までの当院受診患者について,喀痰細菌検査で抗酸菌の分類を実施した.過去3年間にわたる抗酸菌症患者の患者動態,疾患構成を年度毎に検討した.塗抹検査では,蛍光法とキニヨン染色を用い,培養検査には小川培地とMGIT培地を使用した.菌種の同定にはPCR法とDDH法を採用した.細菌検査で抗酸菌が検出されたうち,結核菌が約6割,非結核性抗酸菌(非定型抗酸菌)が4割弱であった.結核菌の割合は上昇する傾向にあった.非結核性抗酸菌で,菌種を同定できたなかでもMAC(Mycobacterium avium-intracellulare complex)が最多であった.
Summary
To investigate the characteristics of mycobacterium infection, we evaluated the classification in patients who consulted the Nishibeppu National Hospital during the period 2000 to 2002. All sputum specimens were tested by smears and by the culture method for mycobacteria. Fluorochrome and Ziehl-Neelsen stain were adopted for the smear method. Liquid media(MGIT) and solid media(Lo¨wenstein-Jensen) were adopted for the culture method.
There are molecular methods that have been developed to identify the type of mycobacteria and detect drug resistance.
About sixty percent showed tuberculosis and about forty percent showed nontuberculous mycobacteria(NTM). The frequency of tuberculosis has been increasing. Among the nontuberculous cases, MAC(Mycobacterium avium-intracellulare complex) with eighty percent, is the most frequently found.
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