IBOTENIC ACID-INDUCED LIMBIC SEIZURES AND NEURONAL DEGENERATION Kazuya Watanabe 1 , Tatsuya Tanaka 1 , Yukichi Yonemasu 1 1Department of Neurosurgery, Asahikawa Medical College pp.505-508
Published Date 1987/6/1
DOI https://doi.org/10.11477/mf.1406205915
  • Abstract
  • Look Inside

Electrographic and clinical observations were made as long as 2 months after the injection of ibo-tenic acid (IBO) solution (50 μg in 1 μl of phos-phate buffer solution) through a chronically implanted cannula into unilateralamygdala of freely moving and non-anesthetized cats. The control group (phosphate buffer group) showed no change during the observation period. About 30 to 60 minutes after the injection of IBO, focal amyg-daloid seizures occurred and propagated to the adjacent limbic structures. Clinically, attention and ipsilateral mydriasis were observed. The siezures occurred only 2 to 5 times and ceased within 4 hours. Cats became electroclinically nor-mal afterwards. Histopathological examination revealed a small necrosis at the injected site of the amygdala. Remarkable pyknosis and gliosis were noted around the necrosis. Remote lesions such as neuronal cell loss and pyknosis were observed in the ipsilateral pyramidal cell layer of the hippocampus. But these changes were mild and not so severe as compared to our previous report of kainic acid microinjection in cats.

Authors emphasized that IBO should be an excellent tool for lesion making and also sug-gested that an aseptic manipulation was essential in the lesion study in cats.

Copyright © 1987, Igaku-Shoin Ltd. All rights reserved.


電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院