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Brain and Nerve No to Shinkei Volume 40, Issue 12 （December 1988）

Brain and Nerve 脳と神経 40巻12号（1988年12月発行）

Japanese English

原著

辺縁系発作の進展に伴う局所脳血流量の変化 REGIONAL CEREBRAL BLOOD FLOW DURING DEVELOPMENT OF LIMBIC SEIZUES INDUCED BY KAINIC ACID (KA) MICROINJECTION INTO UNILATERAL AMYGDALA IN CHRONIC CATS 田中滋也 ¹ , 田中達也 ¹ , 米増祐吉 ¹ Shigeya Tanaka ¹ , Tatsuya Tanaka ¹ , Yukichi Yonemasu ¹ ¹旭川医科大学脳神経外科 ¹Department of Neurosurgery, Asahikawa Medical College pp.1125-1130

発行日 1988年12月1日 Published Date 1988/12/1

DOI https://doi.org/10.11477/mf.1406206217

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Abstract 文献概要
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　抄録　成猫を用いて，カイニン酸を左側扁桃核内に微量注入することにより慢性てんかんモデルを作製した。さらに脳波モニタ—下に，辺縁系発作出現時および回復後での両側扁桃核，左側海馬および左側皮質感覚運動領におけるrCBFを無麻酔，無拘束下に，電気分解による水素クリアランス法により測定した。辺縁系発作出現時のrCBFはcontrol rCBFに比較して，注入側扁桃核で92％，対側扁桃核で60％，注入側海馬で50％，注入側皮質感覚運動領で65％増加したが，辺縁系発作重積状態より回復後には，すべての測定部位でcontrol rCBFに近い値となった。辺縁系発作重積状態より回復後3週を経過すると，注入側扁桃核および海馬で測定したrCBFは軽度低下を示しており，病理学的所見からは神経細胞の脱落に加えて，gliosisが血流低下に関与している可能性があると考えられた。

Regional cerebral blood flow (rCBF) was studied by means of electrolytic hydrogen clearance method in chronically prepared adult cats during develop-ment of limbic seizures induced by KA micro-injection into the unilateral amygdala.

Stereotaxic operation was carried out on 7 adult cats. Bipolar electrodes were placed in the lateral nuclei of bilateral amygdala (LA : left amygdala, RA : right amygdala), and in the ipsilateral dorsal hippocampus (LH). Teflon needles with inner stainless steel needle guides were placed in LA, RA, LH and left anterior sigmoid gyrus (LCx) so as to introduce platinum electrodes for rCBF mea-surements. The teflon needle placed in LA was also utilized for KA microinjection (1 microgram).

Measurements of rCBF were done before (con-trol) and after KA microinjection. Control rCBF were as follows ; LA : 41.6 15.9, RA : 40.7 12.2, LH : 39.1 10.9, LCx : 55.4 20.7 (ml/100 g/min). After KA microinjection, measurements of rCBF were made during limbic seizure stage (LSS) and transfer stage (TS). TS was divided into two stages ; early TS and late TS. In LSS, 50 to 92% increase of rCBF were noted as follows ; LA : 79. 7 23.7, RA : 66.1 18.1, RH : 58.6 17.6, LCx : 91.4 17.8 (ml/100 g/min). In early TS, rCBF in all re-corded sites returned to the same level as control rCBF. In late TS, rCBF in LA and LH were slightly lower than the level of control rCBF.

Pathological examination of LA and LH in early and late TS showed different features. In late TS, astrocytic proliferation was remarkable while it was mild in early TS. These results indicated that the astrocytic proliferation, in addition to neuronal cell loss, was one of major factors in the reduction of rCBF in the epileptic focus.