雑誌文献を検索します。書籍を検索する際には「書籍検索」を選択してください。

Brain and Nerve No to Shinkei Volume 37, Issue 3 （March 1985）

Brain and Nerve 脳と神経 37巻3号（1985年3月発行）

Japanese English

原著

S-adenosyl-L—methionineの実験的脳虚血に及ぼす効果 EFFECTS OF S-ADENOSYL-L-METHIONINE ON EXPERIMENTAL CEREBRAL ISCHEMIA 片山泰朗 ¹ , 清水純 ¹ , 目々澤肇 ¹ , 南澤宏明 ¹ , 稲村憲治 ¹ , 溝口正明 ¹ , 杉本繁 ¹ , 永積惇 ¹ , 赫彰郎 ¹ Yasuo Katayama ¹ , Jun Shimizu ¹ , Hazime Memezawa ¹ , Hiroaki Minamisawa ¹ , Kenji Inamura ¹ , Masaaki Mizoguchi ¹ , Shigeru Sugimoto ¹ , Atushi Nagazumi ¹ , Akiro Terashi ¹ ¹日本医科大学第二内科 ¹Second Department of Internal Medicine, Nippon Medical School pp.243-247

発行日 1985年3月1日 Published Date 1985/3/1

DOI https://doi.org/10.11477/mf.1406205474

PDF(3781KB)

有料閲覧

Abstract 文献概要
1ページ目 Look Inside

　抄録　S-adenosyl-L—methionine・sulfate・tosylate （SAMe）は，細胞膜を構成するリン脂質の主成分であるphosphatidyl cholineの合成促進作用，赤血球の変形能の亢進作用等を持つ。一方，脳損傷に伴って脳内リン脂質の減少することが知られている。従って，これらの作用を持つSAMeは脳虚血の急性期の治療薬として応用されることが期待できる。Wistar kyoto rats （WKY）を用いた（1）心摘出による完全虚血，および自然発症高血圧ラット（SHRSP）を用いた（2）両側総頸動脈閉塞による再開通モデルを使用して，SAMeを投与し，その効果について脳代謝諸量（ATP,lactate,c-AMP），脳含水量を測定して検討した。（1）完全虚血モデルでは，対照群に比してATP,c-AMP値の低下を有意に抑制したが，lactate値には影響を及ぼさなかった。（2）再開通モデルでは，lactateの蓄積を有意に減少させたが，　ATP,c-AMP値には影響を及ぼさなかった。また，脳含水量に対しては効果を認めなかった。以上の結果より，SAMeは脳虚血時および虚血の回復過程において，虚血脳の脳代謝を改善することを示した。

Effects of S-adenosyl-L-methionine (SAMe) on experimental cerebral ischemia were investigated using two different ischemic models. Cerebral energy metabolites (ATP, lactate, c-AMP) andbrain water content were measured.

It is reported that SAMe accelerates synthesis of phosphatydyl choline and increases erythrocyte membrane fluidity.

Complete ischemia was produced by heart exci-sion using wistar kyoto rats. SAMe (100 mg/kg, I. P.) was administered twice at one hour and im-mediately before inducing ischemia. The brain of rats were irradiated by microwave to stop the enzyme activity exactly 60 seconds after inducing ischemia and brain energy metabolites were mea-sured.

Recirculation model was produced by one hour recirculation following two hours ischemia induced by clipping of bilateral common carotid arteries using stroke-prone spontaneously hypertensive rats. SAMe (100 mg/kg, I. V.) was administered twice one hour after clipping and ten miniutes after recirculation. The brain metabolites andwater content were measured one hour after recirculation.

In the complete ischemia, ATP and c-AMP le-vels were statistically high in the SAMe treated group compared to the untreated group (vehicle). But there was no statistical difference in lactate between the treated group and the untreated group.

In the recirculation model, lactate elevation was suppressed in the SAMe treated group compared to the vehicle group with statistical difference, but there was no difference in ATP and c-AMP. Also, there was no difference in water content between the treated and the untreated group.

SAMe protected energy failure in ischemia and accelerated recovery from ischemia. It is indicated that this agent is beneficial for treatment of ce-rebral ischemia in the acute stage.