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Japanese

EFFECTS OF DEXAMETHASONE ON CORTICAL DENDRITIC GROWTH IN MICE Yūji Morikawa 1 , Yasuo Kasubuchi 1 , Masahiro Mino 1 , Hiroshi Yoshioka 1 , Tomoichi Kusunoki 1 , Tsunekazu Yamano 2 , Morimi Shimada 2 1Department of Pediatrics, Kyoto Prefectural University of Medicine 2Department of Pediatrics, Shiga Medical College pp.327-336
Published Date 1978/3/1
DOI https://doi.org/10.11477/mf.1406204221
  • Abstract
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This study was undertaken to investigate the effects of dexamethasone on brain development, with special reference to the cortical dendritic growth with Golgi-Cox method.

The mice of ICR-JCL strain were divided into the control group and the dexamethasone treated group. The latter was subdivided into three groups. The mice in group A were treated with sub-cutaneous injections of dexamethasone 3 mg/kg at 2, 3 and 4 days of age successively. The mice in group B were treated with dexamethasone 5 mg/kg at 5, 6 and 7 days of age, and the mice in group C with 5 mg/kg at 10, 11 and 12 days of age.

The mice treated with dexamethasone were much suppressed in body weight gain and in increase of body and tail length.

The brain weight of the treated mice was also significantly less than that of the control. At 20 days of age, the brains of the experimental animals were 376±17 mg (mean±SD), 362±14 mg, 398±11 mg and 427 ± 18 mg in weight in subgroup A, B, C and the control respectively. The mice in subgroup A and B were significantly less in brain weight than the mice in subgroup C. At 60 days of age, the brains were 443±23 mg and 429±21 mg in weight in subgroup A and B respectively, whereasthey were 492±19 mg in the control. The catch-up growth of the brains in the treated animals was incomplete.

At 20 and 60 days of age, the dendritic spread of the cortical pyramidal neurons in layers IV-V was examined with Golgi-Cox staining in both the treated and the control mice. The dexa-methasone treated mice showed significantly less number of intersections of dendrites with concentric circles drawn at radii of 30 μm, and 60 μm and 80 μm from the center of the perikaryon than the control. Moreover, the mice in subgroup A and Bwere significantly less in number of intersections at radii of 60 μm and 80 μm than the mice in group C and the number of dendrites arising from the perikaryal surface was also significantly less in subgroup A and B than in the control and subgroup C.

In this study the dexamethasone treatment on suckling mouse was proved to inhibit not only the body growth but also the brain development. These facts should be kept in mind in using steroid during infancy, particularly in neonatal period.


Copyright © 1978, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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