雑誌文献を検索します。書籍を検索する際には「書籍検索」を選択してください。

検索

書誌情報 詳細検索 by 医中誌

Japanese

Effects of Gastrointestinal Hormones on the Villus Movements of the Dog R. Nanba 1 1Department of Physiology, Training Institute for Health-teachers, Okayama University pp.1097-1101
Published Date 1975/8/25
DOI https://doi.org/10.11477/mf.1403112364
  • Abstract
  • Look Inside

 When the internal surface of the small intestine is examined under warm saline solution by means of a low-powered microscope, it is seen that each of the villi is extending and contracting. It is generally believed that these movements accelerate the flow of blood and lymph and that they increase absorption.

 Since the discovery of secretin in 1902, the study on the gastrointestinal hormones has developed remarkably. But the relation between the gastrointestinal hormone and the villus movements have yet not been elucidated without villikinin.

 In 1933, Kokas and Ludány introduced the concept of hormonal control by demonstrating that the introduction of hydrochloric acid into the duodenum activated the movements of the villi of a jejunal segment, which was removed from the abdominal cavity and was being viviperfused by anastomosis with the external jugular vein and the carotid artery. Crude mucosal extracts from the upper small intestine were shown to stimulate the movements of the villi, and the active hormone was given the name "villikinin".

 In the present experiments, dogs weighing 8~18 kg were anesthetized by an intravenous injection of pentobarbital sodium (25 mg/kg) and then the abdominal cavity was incised along the midline as previously described by Nanba et al.14)15) perimental method was described in previous paper by Nanba et al.

 As for gastrointestinal hormene, author used cholecystokinin-pancreozymin (Vetrum), tetragastrin (Teikoku Zoki), pentagastrin (Sumitomo Kagaku), glucagon (Sigma), prostaglandins (Ono yakuhin), secresin (Boots pure drug Co. and Sigma chemical Co.) and caerulein (Kyowa Hakko).

 Following Kokas and Ludány's method, author extracted crude villikinin from the duodenum of the dog; the intestinal mucosa of a fasting dog was scraped with a glass slide, weighed, emulsified with sand, and extracted with 0.1 N HCl (30 g mucosa/100 ml HCl). The emulsion was brought to boiling, the pH was adjusted to 7.4~7.5 with 40% NaOH, and the emulsion was cooled. The sediment was removed by centrifugation (2,500 rpm, for 30 min.) and discarded. The supernatant fluid was treated with vegetable charcoal which was removed by suction filtration. This filtrate constituting the crude mucosal extracts or villikinin was frozen and stored at -20℃, or lyophilized.

 The effects of gastrointestinal hormones on the villus movements were studied in anesthetized dogs. The results are summarized as follows.

 1. Serotonin increased remarkably the contractile frequency of the villus movements. Secretin, gastrin and prostaglandins F 2α increased slightly.

 2. Glucagon inhibited remarkably the villus movements.

 3. Cholecystokinin-pancreozymin had no effect and the villikinin showed no effect in the present experiments.


Copyright © 1975, Igaku-Shoin Ltd. All rights reserved.

基本情報

電子版ISSN 1882-1219 印刷版ISSN 0536-2180 医学書院

関連文献

もっと見る

文献を共有