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Radioimmunoassay of Erythropoietin: Analytical Performance and Clinical Use in Hematology pp.578-579
Published Date 1991/6/1
DOI https://doi.org/10.11477/mf.1543900641
  • Abstract

 Erythropoietin (EPO) is the first hormone described as a hematopoietic growth factor, even though Carnot and Deflandre (Fr-Acad Sci 1906; 148: 384) suggested, rather than demonstrated, the activity of the so-called “hematopoietine.” EPO was rediscovered in the USA in the 1950s by Jacobsen et al.; its physiological significance has been objectively studied and its biochemical and genetic identification has been completed. Recently, EPO has been used for clinical trials in some types of severe anemia.

 Determining EPO concentration by bioassays has always beendifficult, long, and expensive: incorporation of 59 Fe into Polycythemic (post-transfusion, or exhypoxic) mice, in vitro incorporation of 59Fein a suspension of liver cells from newborn mice, or in vitro growth oferythrocyte colonies of normal or erythroleukemic cell lines. Theearly radioimmunoassays (RIAs) developed were faulty, mainlybecause of incomplete purification of the antigen.


Copyright © 1991, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1375 印刷版ISSN 0301-2611 医学書院

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