神経細胞内アセチルコリンエステラーゼの細胞学的局在部位の決定

Cytological Localisation of Acetylcholine Esterase in the nerve cells

　Koelle氏Thiocholine法に若干の変改を加える事により，自律神経節神経細胞内のAcetyl-cholinesterase（AChE）の細胞学的分布状態が，従来の標準術式による成績では得られない正確さで，組織学的に観察出来る（Fukuda and Koelle：Fed. Proc.，17：369, 1958；J. Biophys. Biochem. Cytol.，5：433-440, 1959）．

　変改の主要部は　1．標準術式に於けるpre-incubation solution（24％Na₂，SO₄）に切片を浸漬する時間を5乃至10分間に短縮する。

　Standard procedure of thiocholine method ofKoelle for histochemical demonstration of AChEhas been recently modified so as to make possiblemicroscopic observation of neuronal AChE intracellularly.

　Results so obtained were compared with histological preparations such as for neurofibrils, Nissl, Substance Golgi-apparatus, and with intravital methylene blue method of Ehrlich. With the modifiedthiocholine method, under phase-contrast microscope in particular, the cytological distribution ofcopper thiocholine sulphate resembled closely theNissl pattern. The result obtained with materialgiven high doses of irreversible inhibitor, D.F.P., revealed also an identical distribution withincytoplasm of the enzymic product. Furthermore, application of Brachet's methylgreen-pyronin testas combined with the thiocholine's, disclosed bothRNA and copper thiocholine product being virtually superimposed or overlapped one another. They were, however, seen in part to be localizedapart.

　These findings were considered from currentview and literature available to be further supportto the hypothesis that intracellular or internal(reserve) AChE is synthesized within or at theendoplasmic reticulum.