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Disease-specific patterns of protein deposition and their relation to neurodegeneration Toshiki Uchihara 1 1Department of Neuropathology, Tokyo Metropolitan Institute for Neuroscience. Keyword: 萎縮・変性・細胞死 , conformation , 三重染色 , 三次元再構成 pp.334-345
Published Date 2004/6/10
DOI https://doi.org/10.11477/mf.1431100263
  • Abstract
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 Possible relevance of protein deposits, characteristic for some neurodegenerative disorders, were dissected into 1)how the protein is deposited to form inclusions and 2)how neurodegeneration is related to the deposits. Some genetic abnormalities linked to neurodegenerative disorder, for example pathological mutations of tau gene or pathological expansion of CAG repeat, result in these two aspects. Also summarized are technical challenges to realize enhanced multilabeling, three-dimensional reconstruction and how to interpret silver-staining methods. Deposition of protein was investigated on tau in ischemic foci or in glial cytoplasmic inclusions in multiple system atrophy that was found, in both conditions, detectable exclusively with tau2 antibody, whose epitope is not dependent on phosphorylation. This tau2 immunoreactivity was abolished in the presence of detergent(TritonX-100)but restored after washing, suggesting that conformational state of tau2 epitope is dependent on physical and chemical environment and reversible. Difference in the conformational state may distinguish different diseases or different stages of a disease. How neurodegeneration is related to deposits was investigated on CAG repeat disorders relevant to both formation of nuclear inclusions(NI)and neurodegeneration. Cross sectional area of pontine neurons with NI was found larger than that of neurons without NI in a series of CAG repeat disorders and neuronal intranuclear hyaline inclusion disease. Although protein deposition and neurodegeneration is tightly linked to each other, their correlation is not necessarily positive. Independent analyses of these two aspects may clarify their relationship to understand what is going on in human brains with neurodegenerative disorders.


Copyright © 2004, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1243 印刷版ISSN 0001-8724 医学書院

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