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Brain and Nerve No to Shinkei Volume 36, Issue 3 （March 1984）

Brain and Nerve 脳と神経 36巻3号（1984年3月発行）

Japanese English

原著

Developing human spinal cordの免疫組織学的検索—Radial glial cellにおけるvimentin,GFAPの局在について THE IMMUNOHISTOLOGICAL STUDY OF DEVELOPING HUMAN SPINAL CORD:THE LOCALIZATION OF VIMENTINE, GFAP IN RADIAL GLIAL CELL 鎌田一 ¹ , 川合裕 ¹ , 佐藤純人 ¹ , 藤原秀俊 ¹ , 荒清次 ¹ , 小笠原俊一 ¹ , 堀田隆史 ¹ , 中村順一 ¹ , 猿田武 ² , 末松克美 ² Hajime Kamada ¹ , Yutaka Kawai ¹ , Sumito Sato ¹ , Hidetoshi Fujiwara ¹ , Seiji Ara ¹ , Toshikazu Ogasawara ¹ , Takashi Hotta ¹ , Junichi Nakamura ¹ , Takeshi Saruta ² , Katsumi Suematsu ² ¹中村記念病院脳神経外科 ²中村記念病院脳血管研究所 ¹Department of Neurosurgery, Nakamura Memorial Hospital ²Stroke Research Laboratory, Nakamura Memorial Hospital pp.229-235

発行日 1984年3月1日 Published Date 1984/3/1

DOI https://doi.org/10.11477/mf.1406205279

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Abstract 文献概要
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　抄録　ヒト胎児胸髄における，radial glial cellの形態および経時的変化を免疫組職学的に検討した。妊娠齢15週，30週，35週ヒト胎児胸髄および15週ヒト胎児終脳を採取し，凍結切片試料，パラフィン切片試料を作製した。これら試料を抗vimentinマウスモノクロナール抗体ではABC法にて，抗GFAPウサギ血清ではPAP法にて染色した。15週ヒト胎児胸髄では，中心管より軟膜表面へ射状に伸びる，GFAP，vimentinともに陽性の線維を認めた。一部にastrocyteへの初期の分化を示す細胞を認めた。15週ヒト胎児終脳において，vimentin,GFAP陽性の線維を認めた。30週ヒト胎児胸髄ではGFAP,vimentinともに陽性の線維を認めるが，15週に比し屈曲蛇行，分枝化は強化調されていた。更にastrocyteへの分化度の増した細胞を多数認めた。35週胎児胸髄では線維の屈曲蛇行，分枝化は更に増加したがvimentinの染色性は減弱し，成熟度の増したastrocytesを多数認めた。以上のことより，1）ヒトradial glial cellはGFAP，vimentin陽性であること，2） radial glial cellは胎齢の増加により　vimentin　の染色性は減弱すること，3） radial glial cellはastrocyteへ分化すること，4） radial glial cell出現時期は，従来の説より早期であることが示された。

The morphological changes of radial glial cellsin developing human spinal cord have been studied immunohistologically. The specimens extracted from developing human spinal cords, gestational age of 15 weeks, 30 weeks and 35 weeks, were prepared with cryostat for the investigation of anti vimentin mouse monoclonal antibody and of GFAP rabbit serum. We have stained the spe-cimens for vimentin in accordance with ABC method, for GFAP in accordance with PAP me-thod. On gestational age of 15 weeks, the fibers radially oriented from the central canal to pial surface were positive for vimentin and GFAP. Partially, early differentiated astrocytic cells were found. On gestational age of 30 weeks, radi-ally oriented fibers were positive for vimentin and GFAP. They took more tortuous course, bran-ching than those of 15 weeks. More differentiated astrocytic cells were found. On gestational age of 35 weeks, the staining for vimentin was very weak on radially oriented fibers and these fibers were more tortuous, branching than those of 30 weeks. More differentiated astrocytic cells than those of 30 weeks were found in rich amount. From results above, we concluded : 1) Radial glial cells in developing human spinal cord were positive for vimentin and GFAP. 2) They show weaker staining for vimentin in process of gestational age. 3) They differentiate to astrocyte. 4) They appear in earlier stage than suggested by previous reports.