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Vascular Endothelial Growth Factor Expression in the Rat Dural Arteriovenous Fistula Model Yasushi Shin 1 , Ryunosuke Uranishi 1 , Hiroyuki Nakase 1 , Toshisuke Sakaki 1 1Department of Neurosurgery, Nara Medical University Keyword: dural arteriovenous fistula(DAVF) , venous hypertention , vascular endothelial growth factor(VEGF) , rat pp.946-952
Published Date 2003/11/1
DOI https://doi.org/10.11477/mf.1406100547
  • Abstract
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Although various mechanisms of the development of dural arteriovenous fistula (DAVF) have been proposed, the pathogenesis of these lesions are still unclear. Recent experimental evidence suggested a role of angiogenic growth factors in the genesis of vascular malformations of the central nervous system. To further investigate the pathogenesis of DAVF, we examined the expression of the angiogenic growth factor, vascular endothelial growth factor (VEGF), in rat DAVF model.

Material and Methods : Male Wistar rats (weighting 280 to 300g, n=40) were used. Each rat was mounted on a stereotaxic frame under general anesthesia. DAVF model (Spetzler et al.) was made by common carotid artery-external jugular vein anastomosis, bipolar coagulation of the vein draining the transverse sinus, and superior sagittal sinus thrombosis (SSS). SSS was thrombosed by a hemostatic agent through 16-gauge needle. Venous hypertension was induced in 30 rats, which were divided into two experimental groups; (1) immunohistological study group (n=15) and (2) angiography group (n=15). Immunohistological analysis was performed by VEGF antibody 1 week after, and angiography was done 90 days after the surgery. Developing of angiographical DAVF was observed with the magnifying X-ray camera. Each 5 rats served as sham-operated controls, which received a similar surgery without induction of venous hypertension.

Results : VEGF expression and DAVF were not observed in sham group. In immunohistological study group, VEGF expression in the endothelium and the connective tissues of the dura mater in the five rats (33%) and in the neurons in the eleven rats (73%) of the cerebral cortex and the basal ganglia were identified. In angiography group, DAVF formed in 6 among 15 rats (40%).

Conclusion : The findings of this study provide the first experimental evidence that angiogenic growth factors VEGF may participate in the genesis of DAVF. These results suggest a novel strategy for the management and prevention of DAVF and related disorders.


Copyright © 2003, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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