Japanese

Intravital bone imaging ~Osteocyte. Sano Hiroshige 1 , Kondo Naoki 2 , Endo Naoto 3 1Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences/Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Japan/Bone Research Group, Department of Medicine, University of Cambridge, UK. 2Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, Japan. 3Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, Japan. pp.223-230
Published Date 2018/2/28
DOI https://doi.org/10.20837/4201802223
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 Osteocytes are the most abundant cellular component of bone and have been considered dormant until recent evidence has demonstrated their critical roles in bone homeostasis and endocrine regulation. Until now the location of osteocytes within mineralized bone has restricted experimental access, especially in vivo. Here, intravital bone imaging by two-photon excitation microscopy allowed us to directly visualize the osteocytic lacuno-canalicular system. We demonstrated that sciatic neurectomy causes significant acidification around osteocytic lacunae and enlargement of lacuno-canalicular areas. These results show that two-photon intravital microscopy is useful for analysis of osteocytes in vivo.



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電子版ISSN 印刷版ISSN 0917-5857 医薬ジャーナル社

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