Japanese

Evaluation of Bone;Up Date from Research to Clinical Application. Analysis of bone tissues by using fluorescent imaging. Kikuta Junichi 1 , Ishii Masaru 2 1Laboratory of Cellular Dynamics, Immunology Frontier Research Center, Osaka University, Japan Science and Technology, CREST, Japan. 2Laboratory of Cellular Dynamics, Immunology Frontier Research Center, Osaka University, Japan Science and Technology, CREST, Japan. pp.355-360
Published Date 2013/2/28
DOI https://doi.org/10.20837/4201303049
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 Rapid development of fluorescent imaging techniques enables us to understand cellular dynamics in vivo. We have originally established an advanced imaging system for visualizing live bone tissues with intravital multiphoton microscopy. By means of the system we have recently succeeded in visualization of mature osteoclasts in live bones and revealed that RANKL regulates bone-resorptive functions of mature osteoclasts in vivo. We also developed new image analysis software for tracking morphological changes of mature osteoclasts and generated pH-sensing chemical fluorescent probes for detecting bone resorption at local sites on the bone surface. Here we show the latest data and the detailed methodology of intravital imaging of bone tissues, and also discuss its further application.



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電子版ISSN 印刷版ISSN 0917-5857 医薬ジャーナル社

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