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Multiscale Imaging of Neural Activity Using Two-Photon Microscopy Kazuo Kitamura 1 1Department of Neurophysiology, Division of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi Keyword: カルシウムイメージング , カルシウム感受性蛍光蛋白質 , 体積イメージング , 広視野イメージング , calcium imaging , genetically-encoded calcium indicators , volumetric imaging , large field-of-view imaging pp.799-805
Published Date 2024/7/1
DOI https://doi.org/10.11477/mf.1416202686
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Abstract

Two-photon calcium imaging is widely used to observe neural activity in animal brains. Improvements in two-photon microscopy and calcium indicators in recent years have led to higher sensitivity, faster speed, and larger field-of-view imaging, which have facilitated observation of large-scale neuronal activity in three dimensions on a micrometer to millimeter scale. In this paper, we describe these novel two-photon imaging techniques and their applications to neuroscience.


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電子版ISSN 1344-8129 印刷版ISSN 1881-6096 医学書院

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